An Optimization Procedure for Preparing Aqueous CAR/HP-CD Aggregate Dispersions.

Enrika Celitan,Ruta Gruskiene,Jolanta Sereikaite

doi:10.3390/molecules26247562

Enrika Celitan, Ruta Gruskiene + Show 1 more

Open Access

https://doi.org/10.3390/molecules26247562

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Abstract

β-Carotene is a very important molecule for human health. It finds a large application in the food industry, especially for the development of functional foods and dietary supplements. However, β-carotene is an unstable compound and is sensitive to light, temperature, and oxygen. To overcome those limitations, various delivery systems were developed. The inclusion of β-carotene by cyclodextrin aggregates is attractive due to non-toxicity, low hygroscopicity, stability, and the inexpensiveness of cyclodextrins. In this study, β-carotene/2-hydroxypropyl-β-cyclodextrin aggregates were prepared based on the procedure of the addition of β-carotene in an organic solvent to the hot water dispersion of 2-hydroxypropyl-β-cyclodextrin and the following instant evaporation of the organic solvent. The best conditions for the aggregate preparation were found to be as follows: 25% concentration of 2-hydroxypropyl-β-cyclodextrin in water, 65 °C temperature, and acetone for β-carotene dissolution. The efficiency of entrapping was equal to 88%. The procedure is attractive due to the short time of the aggregate preparation.

Highlights

Introduction βCarotene (CAR) belongs to the carotenoid family
CAR is a versatile isoprenoid for human health. It is a precursor of vitamin A, and in humans, dietary β-carotene is cleaved to two molecules of all-transretinal by the action of β, β-carotene 15,15 –monooxygenase 1
CAR/HP-CD aggregates were prepared at different HP-CD concentrations and reaction temperatures using various solvents for CAR dissolution

Summary

Results and Discussion

CAR/HP-CD aggregates were prepared at different HP-CD concentrations and reaction temperatures using various solvents for CAR dissolution. To estimate the amount of CAR entrapped into HP-CD aggregates, the best method for the extraction of CAR from CAR/HP-CD aggregates and transferring CAR into the organic phase must be chosen. Known published methods for the extraction of encapsulated carotenoids were analyzed and tested (Table 1). The best result in terms of extraction completeness was achieved using method 2. This method was further used for the extraction of CAR and the estimation of its amount in the optimization process of the preparation of aqueous CAR/HP-CD aggregate dispersions

Raman Spectroscopy Measurements

Relative Stability of Encapsulated β-Carotene

Conclusions