An Optical Model of Whole Blood for Detecting Platelets in Lens-Free Images

Abstract

In this paper we consider the task of detecting platelets in images of diluted whole blood taken with a lens-free microscope. Despite having several advantages over traditional microscopes, lens-free imaging systems have the significant challenge that the resolution of the system is typically limited by the pixel dimensions of the image sensor. As a result of this limited resolution, detecting platelets is very difficult to do even by manual inspection of the images due to the fact that platelets occupy just a few pixels of the reconstructed image. To address this challenge, we develop an optical model of diluted whole blood to generate physically realistic simulated holograms which we then use to train a convolutional neural network (CNN) for platelet detection. We validate our approach by collecting both lens-free and fluorescent microscopy images of the same field of view of diluted whole blood samples with fluorescently labeled platelets.