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Abstract
A chiral sensor with optical rotation detection based on weak measurement for the kinetic study of sucrose hydrolysis is presented. Based on the polarization modulation to the pre-selection state, the optical rotation of chiral sample was accurately determined through the central wavelength shift of the output spectrum. With this approach, the concentration response curves of sucrose and its hydrolysis products, i.e., fructose and glucose, were experimentally obtained for the hydrolysis analysis. By collecting the output spectrum with a frequency of 100 Hz and fitting the central wavelength shift synchronously during the measurement, the sucrose hydrolysis process was monitored in real time. Different hydrolysis conditions with varied concentration of invertase enzyme and citrate were implemented in this work. As a consequence, the real-time hydrolysis curves of the hydrolysis process with distinct velocities was achieved and analyzed. Such a kinetic monitoring about sucrose hydrolysis with optical rotation detection technology played a critical role in the researches involving sucrose, and also revealed the great potential of weak measurement in intersections, such as food safety inspection and chemical analysis.
Highlights
As the main carbohydrate reserve and energy source in human diet, sucrose exists extensively in plants
In the weak measurement system, the output probe will be reshaped with the action of post selection state
An optical rotation detection system based on weak measurement was built for the study of chiral analysis, especially for the monitoring of sucrose hydrolysis
Summary
As the main carbohydrate reserve and energy source in human diet, sucrose exists extensively in plants. The hydrolysis process of sucrose produces an equimolar monosaccharide mixture, i.e., glucose and fructose. Such a transformation provides a sweetener, which is desirable in food and medicine industries with the advantages of an instant energy source, high osmotic pressure and low crystallization [1]. As a fundamental tool for the monitoring of hydrolysis process, a kinetic measurement technology is significant for the studies involving sucrose hydrolysis. Rapid analytical methods, such as nuclear magnetic resonance (NMR) spectroscopy [5,6] and in situ FTIR spectroscopy [7], have been reported for the kinetic and mechanistic study of sucrose hydrolysis. The real-time response curve of the hydrolysis reaction, which is a fundamental tool for the understanding and controlling of the chemical processes, has not yet implemented
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