An Open-Source Lipid Bilayer Setup for Hands-On Learning of Biophysics

Abstract

Although people are generally interested in how the brain functions, neuroscience education is hampered by a lack of low cost and engaging teaching materials. To address this, we developed an open-source lipid bilayer amplifier which is appropriate for use in introductory courses in biophysics or neurosciences. The amplifier is designed using the common lithographic printed circuit board fabrication process and off-the-shelf electronic components. In addition, we propose a specific design for experimental chambers allowing the insertion of a commercially available polytetrafluoroethylene film. This device can be used in simple experiments in which students monitor the bilayer formation by capacitance measurement and record unitary currents produced by ionophores like gramicidin A. Used in combination with a low-cost data acquisition board this system provides a complete solution for hands-on lessons, therefore improving the effectiveness in teaching basic neurosciences or biophysics.