An Oenothera biennis Cell Cultures Extract Endowed with Skin Anti-Ageing Activity Improves Cell Mechanical Properties.

Sara Ceccacci,Stefania Arciello,Assunta Tortora,Chiara Di Cicco,Adriana De Lucia,Maria Chiara Monti,Danila Falanga,Giovanni Ausanio,Fabio Apone,Annalisa Tito

doi:10.3390/metabo11080527

Sara Ceccacci, Stefania Arciello + Show 8 more

Open Access

https://doi.org/10.3390/metabo11080527

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Abstract

Skin aging is a very well-known process setting a gradual worsening of skin mechanical features due to a decline in the production of the extra-cellular matrix machinery and to a concurrent change in the contraction process. To slow this progression, it is crucial to induce the expression of several proteins able to promote elastic fibers formation and tissue repair. Here, the Oenothera biennis cell culture aqueous extract has been investigated from a chemical point of view and then it was tested in vitro, in cell, and in ex vivo experiments as adjuvant in counteracting skin aging. Accordingly, it has been shown that the Oenothera biennis extract was able, by increasing MYLK gene expression, to promote matrix collagen contraction, actin polymerization, and the production of essential ECM proteins.

Highlights

Skin aging is a very well-known process induced by endogenous and exogenous factors
UPLC-MS/MS analysis of ObHEx was performed and high-resolution spectrometric data were exploited for the chemical characterization using Global Natural Products Social Molecular Networking (GNPS), a web-based mass spectrometry system that aids in the identification and annotation of natural products (NPs) [18]
A GNPS spectral library search and a Feature-Based Molecular Networking (FBMN) job were performed: the first analysis allowed us to identify natural compounds, comparing their MS/MS spectra with those of structurally characterized metabolites, the second one to group related NPs within a network since similar MS/MS fragmentation patterns were exhibited by structurally similar molecules [19]

Summary

Introduction

Skin aging is a very well-known process induced by endogenous and exogenous factors. During senescence, all the cutaneous physiological functions inexorably degenerate, and this progressive deterioration damages the skin [1]. The most important dermis extra-cellular matrix components are proteins such as collagen I and III, laminin, periostin, tenascin, elastin, fibronectin, and proteoglycans, as their relative amount and folding state play a key role in the interaction between the cells and the matrix, guaranteeing the proper texture of the dermis [3]. Actin cytoskeleton assembly is linked to the cell movement and ability to contract, and to the production of ECM matrix protein through the activation of TGFβ-II receptor (TGFBRII) [9]. This down-regulation in turn decreases the production of collagen and other ECM proteins, resulting in a loss of dermal mass and skin fragility

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