Abstract
A near infrared-emitting cyanine with a very large Stokes shift (Δλ ≈ 230 nm) was synthesized by coupling with excited state intramolecular proton transfer (ESIPT). Fluorescence response of the probe was examined at different pH and at different temperatures, which revealed the important role of ESIPT in the fluorescence. In comparison with commercial FM1-43 (ϕfl ≈ 0.03 in CH2Cl2), which is a popular styryl dye for membrane staining, the developed new probe 2a exhibited a significantly higher quantum yield (ϕfl ≈ 0.34 in CH2Cl2), attributing to the effective intramolecular hydrogen bonding which is present in the latter. In addition, the probe exhibited very good selectivity for imaging plasma membrane of prokaryotic cells, indicating its potential for imaging membrane of procaryotic (E. coli) cells.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
