Abstract
Aims: Breast cancer is the most common type of cancer among women and ranks second among the causes of female death in the world. In order to find a solution to breast cancer, different studies are being conducted for the treatment and the effects of different drugs and substances on this disease are intensively investigated. Boric acid has been shown to control the proliferation of certain types of cancer cells.Noscapine is one of the ingredients in Papaver somniferum (opium). It was first isolated from Papaver somniferum (opium) in 1817. It is one of the most abundant opioids found in the opium plant (up to 10% of the total composition) after morphine. It is also known as Narcotine, Nectodon, Nospen, Anarcotine, and (archaic) Opiane and occurs in the (-)α isomer which has S, R stereochemistry (S stereochemistry at phthalide-carbon and R at isoquinoline-carbon). Noscapine is structurally and chemically different from other opium alkaloids such as morphine, codeine, thebaine, papaverine, and narceine.
 Materials and Methods: Based on this information, this study was conducted in vitro to optimize the pure form of noscapine (obtained from the poppy capsule) by applying different concentrations on MCF-7 breast cancer cell lines. HPLC technique which is one of the most widely analytical techniques has been used in this study. Determination of the LD50 value and cell proliferation by viability test was also performed to investigate the predicted effects of noscapine on MCF-7 breast cancer cell lines using VEGF (Vascular Endothelial Growth Factor) and PARP (Poly (ADP-Ribose) Polymerase) Analysis.
 Discussion: According to the results, it was observed in proliferation experiment that the vitality values decreased in direct proportion to the concentration and time at concentrations of 5 ppm, 10 ppm, 25 ppm, 50 ppm, 75 ppm, and 100 ppm. The LD50 value was determined as 50 ppm. There was no significant difference in VEGF values. It was also observed that the PARP level was lower than control group.
 Conclusion: As a result of the vitality test performed with the CCK-8 kit, it was determined that noscapine has an antiproliferative effect in various concentrations. The low PARP data in the noscapine groups suggests that the cell goes to apoptotic death.
Highlights
As a result of the vitality test performed with the CCK-8 kit, it was determined that noscapine has an antiproliferative effect in various concentrations
The main content of the medium used in the culture of MCF-7 human breast adenocarcinoma cells is RPMI 1640, and it contains 10% fetal medium was added to the cells separated from bovine serum (FBS) and 1% penicillin / the surface to eliminate the effect of trypsin
Poly (ADP-Ribose) Polymerases (PARPs) and VEGF analyzes were performed on MCF-7 cell line treated with noscapine at 25 ppm, 50 ppm and 100 ppm concentrations
Summary
The main content of the medium used in the culture of MCF-7 human breast adenocarcinoma cells is RPMI 1640, and it contains 10% fetal medium was added to the cells separated from bovine serum (FBS) and 1% penicillin / the surface to eliminate the effect of trypsin. Seeded in a 96-well plate and allowed to incubate at 37°C for 60 minutes. Cells were removed with the aid of trypsin After that, they were centrifuged and the supernatant was discarded and the resulting pellet was turned into suspension with the addition of medium. The stand in the incubator for 24 hours .Following cells in the eppendorf tube were centrifuged at incubation; the cells were treated with various 2100 rpm for 15 minutes at 15oC. After 48 hours, The medium was added to the eppendorf tube the cells were removed from the incubator. Prepared samples and standards were trypsin was added to the cells and placed in a seeded in a 96-well plate and allowed to incubate. Chromogen solution A and B were added and incubation at 37°C for 10 minutes was allowed. The stop solution was added and the measurement was performed
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