An MBoC Favorite: Identification of novel principles of keratin filament turnover in living cells

Abstract

Molecular Biology of the CellVol. 23, No. 20 MBoC 20th Anniversary FavoritesFree AccessAn MBoC Favorite: Identification of novel principles of keratin filament turnover in living cellsPierre A. CoulombePierre A. CoulombeSearch for more papers by this authorPublished Online:13 Oct 2017https://doi.org/10.1091/mbc.e12-02-0160AboutSectionsView PDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmail In celebration of MBoC's first 20 years, members of the Editorial Board, members of the ASCB Council, and others comment on their favorite MBoC papers from the past two decades.This study examines the attributes of keratin intermediate filaments in single, live, nonmotile epithelial cells under steady-state conditions. From their substantive findings, the authors proposed a spatially and temporally defined “keratin cycle” that accounts for the genesis, maturation, and turnover of keratin filaments in the setting of live epithelial cells in culture. I suspect that, just like me, many researchers did not expect that the process of assembly to turnover of keratin filaments would be so vectorial and spatially well-defined, even if it ultimately applies only to a cell culture setting. The “keratin cycle” also provided a conceptual framework from which to examine the relationships among keratin filaments, F-actin, microtubules, and adhesion complexes. The model has been refined since, and several of the predictions it originally generated have been upheld through subsequent experimentation. Finally, this study provided an alternative view of intermediate filament dynamics at steady state, complementing similar efforts from other researchers.FOOTNOTESmbc.E12-02-0160Molecular Biology of the Cell Volume 23 Page 3926.REFERENCE Windoffer R, Woll S, Strnad P, Leube RE (2004). Identification of novel principles of keratin filament turnover in living cells. Mol Biol Cell 15, 2436-2448. Link, Google ScholarFiguresReferencesRelatedDetails Vol. 23, No. 20 October 15, 20123925-4142 Metrics Downloads & Citations Downloads: 33 History Information© 2012 Coulombe. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).PDF download