Abstract
Molecular Biology of the CellVol. 23, No. 9 MBoC 20th Anniversary FavoritesFree AccessAn MBoC Favorite: Ca2+ entry through store-operated channels in mouse sperm is initiated by egg ZP3 and drives the acrosome reactionPaul M. WassarmanPaul M. WassarmanSearch for more papers by this authorPublished Online:13 Oct 2017https://doi.org/10.1091/mbc.e12-02-0147AboutSectionsView PDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmail In celebration of MBoC's first 20 years, members of the Editorial Board, members of the ASCB Council, and others comment on their favorite MBoC papers from the past two decades.To fertilize eggs, mammalian sperm must first undergo a form of cellular exocytosis called the acrosome reaction. In 1983, my laboratory reported that nanomolar concentrations of ZP3, one of three zona pellucida glycoproteins from mouse eggs, induced mouse sperm to undergo the acrosome reaction in vitro. Seven years later, this article from Florman's laboratory (O'Toole et al., 2000) revealed that when ZP3 binds to sperm heads, it generates a sustained influx of Ca2+ through store depletion–activated channels that function to refill internal Ca2+ stores and triggers the acrosome reaction. In the presence of egg ZP3, Ca2+ levels in sperm increased nearly 2.5-fold, from ∼159 to ∼396 nm. This article provided a comprehensive model for the ionic events responsible for ZP3-induced signal transduction in sperm during mammalian fertilization.FOOTNOTESDOI: 10.1091/mbc.E12-02-0147Molecular Biology of the Cell Volume 23 Page 1605.REFERENCE O'Toole A, Arnoult C, Darszon A, Steinhardt R, Florman HM (2000). Ca2+ entry through store-operated channels in mouse sperm is initiated by egg ZP3 and drives the acrosome reaction. Mol Biol Cell 11, 1571-1584. Link, Google ScholarFiguresReferencesRelatedDetails Vol. 23, No. 9 May 01, 20121605-1796 Metrics Downloads & Citations Downloads: 39 History Information© 2012 Wassarman. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).PDF download
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