Abstract
This is the first report describing an analytical method for quantitative analysis of two naturally occurring sulphur compounds, S-methyl-l-cysteine (SMC) and S-methyl-l-cysteine sulfoxide (SMCSO), in human body fluids using isotope-labelled internal standards and liquid chromatography-mass spectrometry (LC-MS)/MS techniques. This method was validated according to the guideline of the Royal Society of Chemistry Analytical Methods Committee. It offers significant advantages including simple and fast preparation of human biological samples. The limits of detection of SMC were 0.08 µM for urine and 0.04 µM for plasma. The limits of detection of SMCSO were 0.03 µM for urine and 0.02 µM for plasma. The calibration curves of all matrices showed linearity with correlation coefficients r2 > 0.9987. The intra and inter day precisions in three levels of known concentrations were >10% and >20%, respectively. The quantification accuracy was 98.28 ± 5.66%. The proposed method would be beneficial for the rapid and accurate determination of the SMC and SMCSO in human plasma and urine samples using by isotope labelled internal standards.
Highlights
Dietary sulphur compounds are of potential value in protecting humans against chronic diseases [1–3]
There is growing interest in the bioactivity of S-methyl-l-cysteine sulfoxide (SMCSO) [6,7,8,9], which is mainly found in Allium species [10,11,12]
Samples obtained from we report the development and validation of a rapid, highly sensitive liquid chromatography-mass spectrometry (LC-MS)/MS method without large dietaryderivation intervention studies for better understanding the pharmacokinetics of these steps to enable the identification and quantification of SMC and SMCSO in body fluids. compounds in humans
Summary
Dietary sulphur compounds are of potential value in protecting humans against chronic diseases [1–3]. Dietary sulphur compounds are of potential value in protecting humans against chronic diseases [1–. The literature is rich in studies supporting the anticancer properties of cruciferous vegetables. These are uniquely characterised by high levels of sulphur-delivering compounds such as isothiocyanate precursors [4,5]. To understand the association between SMCSO intake from diets and its potential benefits for human health, evidence-based knowledge on its bioavailability, including tissue distribution, is required. Several analytical methods have been reported for the analysis of SMCSO in plant materials [15,16,17,18,19,20,21,22], but there are only a few reports on samples of human origin [14,23]
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