Abstract
Abstract Androgen actions in the rat prostate gland are complicated because of extensive metabolism of the androgens. Traditional methods for separating and quantitating the androgens and their metabolites involved thin layer and paper chromatographic steps which are laborious and time-consuming. An isocratic reversed-phase high performance liquid chromatographic system has been developed which separates 11 androgens. This system has been applied to the analysis of the androgen metabolites formed by rat ventral prostate cells in culture and these compounds quantitatively account for all significant metabolism. Using a flow-through radioactivity detector experiments are performed with 3H-substrates. Previously reported systems did not achieve separation of all the compounds.
Published Version
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