Abstract
An isocratic liquid chromatographic method for the separation and simultaneous determination of retinyl acetate, propionate or palmitate (esters of vitamin A), β-carotene (pro-vitamin A), ascorbic acid (vitamin C) and dl-α-tocopherol (vitamin E) is described. Samples are analysed by means of a reversed-phase column (LiChrospher 100 RP-18), using methanol as mobile phase. The UV–Vis detector used was set at a wavelength of 300 nm and switched to 450 nm at 17 min, allowing the determination of β-carotene. These vitamins were separated within 25 min and the detection limits ranged from 7 (β-carotene) to 65 ng ml −1 (ascorbic acid).
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