Abstract
The insertion sequence IS 900 has been considered specific for Mycobacterium avium subsp. paratuberculosis ( M. paratuberculosis) and has, therefore, been used as the target gene for diagnostic PCR of M. paratuberculosis. From a healthy dairy cow we have isolated and characterised a mycobacterium harbouring one copy of a sequence with 94% identity to IS 900 at the nucleic acid level. The isolate was shown to be related to Mycobacterium cookii, as assessed by 16S rRNA sequencing. Strong amplifications were obtained with several PCR primers described for detection of IS 900. This finding shows the need of alternative PCR systems based on other genes than IS 900 to confirm the presence of M. paratuberculosis.
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