An investigation on the chloroplast and nuclear genomes of taxa belong to the subgenus Dracunculus (Bess.) Rydb. of Artemisia L. (Asteraceae) in Turkey.

P Yilmaz Sancar,S Civelek,C F Wahi D,M Kursat

doi:10.1590/1519-6984.242403

P Yilmaz Sancar, S Civelek + Show 2 more

Open Access

https://doi.org/10.1590/1519-6984.242403

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Abstract

Artemisia is one of the biggest genera in the family Asteraceae, with around 500-600 taxa at specific and sub-specific levels and organised in 5 subgenera. Due to the high number of taxa, a lot taxonomists are trying to solve the problem of its classification and phylogeny but its natural classification still hasn't been achieved. In this research, 60 individuals belonging to 4 taxa of the subgenus Dracunculus of Artemisia L. in Turkey were examined. For all the examined individuals from both the same and different populations belonging to the taxa of the subgenus Dracunculus, the sequences of the regions both psbA-trnH of chloroplast DNA and ITS of nuclear DNA were determined. Also, the gene regions obtained were recorded in the NCBI GenBank database and an accession number was taken. It was found that there was no gene flow and hybridization between the four studied taxa of the subgenus Dracunculus, and these 4 taxa also completed their speciation. According to the results of this molecular study, A. campestris var. campestris, A. campestris var. marschalliana and A. campestris var. araratica were proposed to be raised from the variety level to the species level. This research is important as it is the first molecular based study relating with the subgenus Dracunculus growing in Turkey.

Highlights

IntroductionArtemisia L. is one of the larger genera in the family Asteraceae and the largest genus in the tribe Anthemideae, and comprises of 200 to more than 500 taxa at the specific or subspecific level (McArthur, 1979; Mabberley, 1987; Ling, 1991a; Ling, 1991b; Bremer and Humphries, 1993; Ling, 1995a; Ling, 1995b; Torrell et al, 1999).The genus Artemisia L. contains all life forms except trees: Annual, biennial and perennial herbs, suffruticoses (subshrubs), fruticose (shrubs), with some large in stature.The genus Artemisia in the Flora of Turkey are not divided to sections or subgenera
In this study a phylogenetic systematic study was conducted by using the molecular data of subgenus Dracunculus that grow in Turkey. 60 individuals of 21 specimens taken from 17 different populations belonging to 4 taxa of the subgenus Dracunculus were examined by analyzing the base slice of the regions being obtained, in an attempt to find some information about the closeness and distance of the taxa with each other
The phylogenetic relationship among the different Artemisia species collected from different regions of Pakistan based on the chloroplast gene RPS11 was investigated by Mahmood et al (2011)

Summary

Introduction

Artemisia L. is one of the larger genera in the family Asteraceae and the largest genus in the tribe Anthemideae, and comprises of 200 to more than 500 taxa at the specific or subspecific level (McArthur, 1979; Mabberley, 1987; Ling, 1991a; Ling, 1991b; Bremer and Humphries, 1993; Ling, 1995a; Ling, 1995b; Torrell et al, 1999).The genus Artemisia L. contains all life forms except trees: Annual, biennial and perennial herbs, suffruticoses (subshrubs), fruticose (shrubs), with some large in stature.The genus Artemisia in the Flora of Turkey are not divided to sections or subgenera.

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