Abstract
This study aimed to investigate the effects of Extracellular Vesicles (EV) secreted from mouse embryonic fibroblasts EV on wound healing of full-thickness skin defect in a diabetic mouse model. The study included both in vitro and in vivo experimental studies 82 mice. In the in vitro stage of the experimental study, hysterectomy was performed on two mice between the 13-14th days of pregnancy and then EV was isolated by cell culturing. VEGF, IL-6, and TNF-α biomarkers were examined in tissue homogenate. Moreover, tissue taken from wound area was also subjected to histopathologic scoring. EV augmented the effect of VEGF. Therefore promoted angiogenesis increases the transport of cells, essential oxygen and nutrients in the wound area. Extracellular vesicles isolated from mouse embryonic fibroblasts have been found to accelerate wound healing in diabetes. The findings obtained from this experimental study indicate that EV isolated from mouse embryonic fibroblasts accelerate the wound healing process in experimentally induced-diabetes in mice.
Highlights
Fibroblasts are the primary cells of connective tissues, which are relatively undifferentiated mesodermal cell types abundantly found in the connective tissue and they play a role in various processes of wound healing (Rodero & Khosrotehrani 2010)
EFFECT OF EXTRACELLULAR VESICLES ON WOUND HEALING. In light of this information, this study aimed to investigate the role of Extracellular Vesicles (EV) isolated from Mouse embryonic fibroblast cells (MEFs) in wound healing in an experimental diabetic mouse model and the level of İL-6, TNF-α, and VEGF biomarkers in relevant tissues
According to the figure 2 comparing the wound area according to days in mice in the C, C+ EV, DC, and D+EV groups in which a full-thickness skin defect was created, there was no significant difference on day 1, when the said skin defect was created (p>0.05)
Summary
Fibroblasts are the primary cells of connective tissues, which are relatively undifferentiated mesodermal cell types abundantly found in the connective tissue and they play a role in various processes of wound healing (Rodero & Khosrotehrani 2010). One of the critical factors released from EV isolated from MEFs is the Fibroblast Growth Factor (FGF). FGFs are structurally related mitogens that regulate the differentiation of various cells. In addition to promoting growth, FGFs are thought to play various roles in controlling cellular differentiation in a wide variety of cell types. Wound healing is an extremely complicated process that involves a series of complex dynamic events in several stages (Hormozi et al 2017). Wound healing is a complex process that consists of integrated stages such as inflammation, granulation tissue formation, re-epithelialization, matrix formation, and remodeling (Barrientos et al 2008)
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