Abstract
In this study; orotic acid levels in the milk of smoking and non-smoking mothers were investigated by high-performance liquid chromatography (HPLC). It was found that the amount of orotic acid in the milk of smoking mothers (3.92+/-0.20 micro g/ml) was higher than that of non-smoking mothers (1.66+/-0.15 micro g/ml). Orotic acids levels in the milk of smoking mothers in comparison with non-smokers were found to be statistically significant (P<0.005). Smoking may have increased the orotic acid levels by affecting pyrimidine biosynthesis pathway.
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