An investigation of electrical breakdown of bimolecular lipid membranes

Abstract

The investigation is concerned with the irreversible electrical breakdown of bimolecular lipid membranes, depending on the velocity of linear voltage scanning. It was found that the membrane breakdown potential depended on the velocity of electric field variation. For instance, at voltage scanning velocities of up to 0.1 V/s, the rupture of membrane from glycerol monooleate occurs at 0.20–0.25 V and, at velocities higher than 1 V/s, at 0.5–0.6 V. Then the film breakdown depending on lipid phase transition was studied. At high velocities of imposed voltage scanning, the disruption of the bimolecular lipid membranes was shown not to depend on their phase states; at the same time, at low velocities, one could note a slight difference in the stability of the films at temperatures higher and lower than those of the phase transition. Whereas transition from gel to liquid-crystalline state involves transition from an ordered to a less ordered membrane structure with a sharp increase in the number of defects in the membrane, the authors, conclude that the film breakdown in the second case occurs by the ‘defect’ mechanism suggested earlier. It was also assumed that, in certain cases involving low velocities of voltage scanning, membrane breakdown may occur because of variation in the interfacial tension and in the contact angle between the film and torus. Possible mechanisms of the membrane irreversible electrical breakdown at high velocities of voltage variation are discussed. It was shown that breakdown should occur as a result of membrane compression in an electric field by a mechanism previously examined. The elastic moduli of a number of membranes were calculated by the breakdown criterion suggested earlier. They were found to coincide with the results of other investigators and, depending on the type of lipid, to equal 10 5–10 6 Pa.