An investigation of DNA fragmentation and morphological changes caused by bacteria and fungi in human spermatozoa

Abstract

ObjectiveThe main aim of this study verifies the prevalence of semen Bacterial contamination and whether the contamination could cause the DNA fragmentation towards decreased sperm quality.Materials and MethodsRequirements -Glass wares, Toludine blue staining, Papanicolaus staining. Culture media, Semen.Results30 semen specimens to assess the sperm DNA fragmentation. Out of 30 samples, 20 samples were found to have defective DNA. Out of 30 semen samples, 17 samples were found to have defective sperm morphology (Head, mid piece, cytoplasmic droplet and tail) .All the 30 semen specimens were plated on culture media like blood agar. Nutrient agar, macconkey agar and sabourauds dextrose agar. It showed bacterial growth on 20 out of 30 semen samples.An in-vitro study of isolated Candida albicans from the semen, the resultsshowed the attachment of Candida albicans to spermatozoa through head.ConclusionIn conclusion, Candida albicans did not affect sperm parameters, but increased sperm chromatin packaging damage and apoptosis that might have caused fertilization failure after assisted reproduction treatment in this coulples. The DNA fragmentation and morphological changes of the spermatozoa might adversely affect the reproductive outcomes.Burrello,N., A.E.Calogero, A.Perdichizzi, M.D.Salmeri, R.Agata and Vicari,E. 2004. Inhibitation of Oocyte fertilization by assisted reproductive techniques and increased sperm DNA fragmentation in the presence of Candida albicans, a case report, Reprod. Biomed. online.8(5):569-73. ObjectiveThe main aim of this study verifies the prevalence of semen Bacterial contamination and whether the contamination could cause the DNA fragmentation towards decreased sperm quality. The main aim of this study verifies the prevalence of semen Bacterial contamination and whether the contamination could cause the DNA fragmentation towards decreased sperm quality. Materials and MethodsRequirements -Glass wares, Toludine blue staining, Papanicolaus staining. Culture media, Semen. Requirements -Glass wares, Toludine blue staining, Papanicolaus staining. Culture media, Semen. Results30 semen specimens to assess the sperm DNA fragmentation. Out of 30 samples, 20 samples were found to have defective DNA. Out of 30 semen samples, 17 samples were found to have defective sperm morphology (Head, mid piece, cytoplasmic droplet and tail) .All the 30 semen specimens were plated on culture media like blood agar. Nutrient agar, macconkey agar and sabourauds dextrose agar. It showed bacterial growth on 20 out of 30 semen samples.An in-vitro study of isolated Candida albicans from the semen, the resultsshowed the attachment of Candida albicans to spermatozoa through head. 30 semen specimens to assess the sperm DNA fragmentation. Out of 30 samples, 20 samples were found to have defective DNA. Out of 30 semen samples, 17 samples were found to have defective sperm morphology (Head, mid piece, cytoplasmic droplet and tail) .All the 30 semen specimens were plated on culture media like blood agar. Nutrient agar, macconkey agar and sabourauds dextrose agar. It showed bacterial growth on 20 out of 30 semen samples.An in-vitro study of isolated Candida albicans from the semen, the resultsshowed the attachment of Candida albicans to spermatozoa through head. ConclusionIn conclusion, Candida albicans did not affect sperm parameters, but increased sperm chromatin packaging damage and apoptosis that might have caused fertilization failure after assisted reproduction treatment in this coulples. The DNA fragmentation and morphological changes of the spermatozoa might adversely affect the reproductive outcomes.Burrello,N., A.E.Calogero, A.Perdichizzi, M.D.Salmeri, R.Agata and Vicari,E. 2004. Inhibitation of Oocyte fertilization by assisted reproductive techniques and increased sperm DNA fragmentation in the presence of Candida albicans, a case report, Reprod. Biomed. online.8(5):569-73. In conclusion, Candida albicans did not affect sperm parameters, but increased sperm chromatin packaging damage and apoptosis that might have caused fertilization failure after assisted reproduction treatment in this coulples. The DNA fragmentation and morphological changes of the spermatozoa might adversely affect the reproductive outcomes.