Abstract
Supercritical fluid chromatography with both ESI (+) and ESI (-) MS along with photodiode array detection was used to separate and analyze cannabinoids and parent carboxy constituents in 30 cannabis samples. The analytical results were compared to those obtained using a validated UHPLC method, in which, there was an agreement between these two methods within ± 13% for Δ9-THC. The same SFC method was used to study the decarboxylation of Δ9-tetrahydrocannabinolic acid A (THCA-A), cannabidiolic acid (CBDA) and cannabigerolic acid (CBGA) over a range of time (0 – 60 min) and temperature (80, 95, 110, 130 and 145 °C). The reactions at 80 and 95 °C over the full time scale, and at 110 and 130 °C up to the time when decarboxylation was complete, displayed an exponential relation between concentration and time indicating a first-order or pseudo-first-order reaction [1]. The rate constants for THCA-A were twice those of the other two acids, viz., CBDA and CBGA. Moreover, the decarboxylation reaction for THCA-A was forthright, with no side reactions or byproducts. The decarboxylation reactions for CBDA and CBGA were not as distinctly clean purportedly due to evaporation or unexplained loss of reactants or products at high temperatures.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
