Abstract
Abstract. Modern strains of ducks exhibit excessive body fat deposition, which is one of the main problems encountered by duck industry today. Excess fat deposition has led commercial breeders to incorporate significant selection for reduced body fatness in breeding programs. Peroxisome proliferators-activated receptors (PPARs) belong to the nuclear receptor superfamily (SCHOONJANS et al., 1996). In particular peroxisome proliferators-activated receptor gamma, PPARG has been implicated in the regulation of lipid metabolism and, consequently, in diseases that involve disturbances in lipid metabolism (obesity, diabetes, atherosclerosis, cardiac failure) (VAN et al., 2002). Thus PPARG is a candidate gene for traits related to fat deposition.
Highlights
Modern strains of ducks exhibit excessive body fat deposition, which is one of the main problems encountered by duck industry today
SNP identification, genotyping and association analysis: The populations used for identification of SNP in PPARG intron-1 were Z2, Z4 and hybrid lines of Peking duck and the Cherry Valley duck
WU et al.: An intronic SNP of PPARG and its association with fat traits in four meat-type duck populations were measured at 42 days of age after slaughtered
Summary
Modern strains of ducks exhibit excessive body fat deposition, which is one of the main problems encountered by duck industry today. Procedures: Primer sequences: The duck PPARG intron-1 (GenBank accession No EU168847) gene sequence was used to design 3 sets of PCR primers for SNP discovery and genotyping. PCR reactions were carried out in a total volume of 15 μL with 40 ng of genomic DNA, 5 μM of each of forward and reverse primer, 1.5 μL of 10 × buffer, 1.5 mM of MgCl2, 0.25 mM of dNTP, and 1.5 U of Taq DNA polymerase.
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