Abstract
Previous attempts by several groups to clone fragments containing intron 2 of the rat preprotachykinin-A gene have generated deletions of various sizes. We have determined that these deletions occur within a specific region of the intron spanning a CCCT tandem repeat domain. We show that this intronic domain is able to support reporter gene expression in mouse embryonic stem (ES) cells that have been induced to differentiate but not in undifferentiated ES cells. No significant expression was observed in the HeLa clonal cell line. This demonstrates that this intron 2 domain is a highly restrictive enhancer and may be associated with differentiation.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
