Abstract
The tandemly repeated multigene families encoding the 5S and 18S-25S ribosomal RNAs were studied at the restriction enzyme level in Tolmiea menziesii, Tellima grandiflora, and in a putative intergeneric hybrid. Using restriction endonucleases that cut once per repeat, the repeat lengths of the 5S and 18S-25S ribosomal genes were estimated. The 5S ribosomal gene repeat length is approximately 480 and 450 base pairs, respectively, in Tolmiea and Tellima. The repeat length of the 18S-25S ribosomal genes varied from 11–13 kb in Tolmiea, and was only about 9 kb in Tellima. The putative hybrid combined the repeat lengths of both Tolmiea and Tellima for both the 5S and the 18S-25S ribosomal genes. These data substantiate the occurrence of natural hybridization between Tolmiea and Tellima. For both the 5S and 18S-25S gene experiments, the hybrid appears to contain fewer repeats corresponding to Tolmiea than to Tellima.
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