Abstract

Synthetic dyes pose a large threat to the environment and consequently to human health. Various dyes are used in textile, cosmetics, and pharmaceutical industries, and are released into the environment without any treatment, thus adversely affecting both the environment and neighboring human populations. Several existing physical and chemical methods for dye degradation are effective but have many drawbacks. Biological methods over the years have gained importance in the decolorization and degradation of dye and have also overcome the disadvantages of physiochemical methods. Furthermore, biological methods are eco-friendly and lead to complete decolorization. The mechanism of decolorization and degradation by several bacterial enzymes are discussed in detail. For the identification of ecologically sustainable strains and their application at the field level, we have focused on bioaugmentation aspects. Furthermore, in silico studies such as molecular docking of bacterial enzymes with dyes can give a new insight into biological studies and provide an easy way to understand the interaction at the molecular level. This review mainly focuses on an integrative approach and its importance for the effective treatment and decolorization of dyes.

Highlights

  • Dyes have been widely used and considered for their coloring property in different industries

  • The major environmental concerns include the biomagnification of toxic dyes at different trophic levels in the food chain and increased value of biological oxygen demand (BOD) as well as chemical oxygen demand (COD), disturbing the aquatic life (Lellis et al, 2019)

  • Flavoprotein and enzyme flavin reductase or NAD(P)H:flavin oxidoreductase is used (Ingelman et al, 1999). This enzyme catalyzes the flavoproteins into the reduced flavins [flavin denine dinucleotide (FAD) or flavin mononucleotide (FMN)] with the help of re-oxidation of nicotinamide adenine dinucleotide (NADH) or nicotinamide adenine dinucleotide phosphate (NADPH), which are in the reduced form

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Summary

An Integrative Approach to Study Bacterial Enzymatic Degradation of Toxic Dyes

Arti Mishra1*, Simran Takkar[1], Naveen Chandra Joshi[1], Smriti Shukla[2], Kartikeya Shukla[3], Anamika Singh[4], Anusha Manikonda[5] and Ajit Varma[1]. Various dyes are used in textile, cosmetics, and pharmaceutical industries, and are released into the environment without any treatment, adversely affecting both the environment and neighboring human populations. Several existing physical and chemical methods for dye degradation are effective but have many drawbacks. Biological methods over the years have gained importance in the decolorization and degradation of dye and have overcome the disadvantages of physiochemical methods. Biological methods are eco-friendly and lead to complete decolorization. The mechanism of decolorization and degradation by several bacterial enzymes are discussed in detail. In silico studies such as molecular docking of bacterial enzymes with dyes can give a new insight into biological studies and provide an easy way to understand the interaction at the molecular level. This review mainly focuses on an integrative approach and its importance for the effective treatment and decolorization of dyes

INTRODUCTION
ECOTOXICITY BASED ON THE CHEMICAL CHARACTERIZATION OF AZO DYE
Physiochemical Reduction Methods
Electrokinetic coagulation
Sludge generation
Nanobiotechnology and Innovative Approaches
Biological Reduction Methods
Bacterial consortium YHK
Bioaugmentation of Dye Toxicity
Extrinsic Factors
Intrinsic Factors
Nicotinamide Adenine
Riboflavin Reductase
Lignin Peroxidase
IN SILICO STUDIES OF INTERACTIONS OF DYES WITH BACTERIAL ENZYMES
ECOLOGICAL IMPORTANCE OF DETOXIFICATION OF AZO DYES BY MICROORGANISMS
Cellular and Molecular Toxicity
Chronic Toxicity and Phytotoxicity
CONCLUSION AND FUTURE PERSPECTIVE
Findings
AUTHOR CONTRIBUTIONS
Full Text
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