An integrative analysis of Vitis vinifera L. NAC genes in response to Botrytis cinerea

Abstract

Botrytis cinerea, a pathogenic necrotizing fungus, induces diseases in grapevines such as grey mold, pre- and post-harvest decay, and a reduction in fruit quality. Plant biologists focus on Vitis vinifera L. NAC genes (VvNAC) due to their crucial role in the plant defense system against diverse stresses. The objective of this study was to conduct an integrative analysis of VvNAC genes, investigating their responses to B. cinerea through an in silico method followed by an in vitro approach. The goal was to establish a connection between the outcomes of both approaches, potentially offering complementary insights. The in silico approach involved the characterization of 74 VvNAC genes within the grapevine's genome. This encompassed the identification of subcellular localization, transmembrane helices, evolutionary rates, promoter analysis, protein-protein interactions, and expression patterns in response to B. cinerea infection followed by a quantitative real-time PCR (qRT-PCR). A noteworthy discovery from our analysis revealed that 10 different VvNAC genes have been linked to B. cinerea tolerance. This finding has the potential to serve as a model for future breeding programs.