Abstract

We have evaluated the effect of an integrated (nano-bio) technique involving the use of stabilized Pd/Fe0 bimetallic nanoparticles (CMC-Pd/nFe0) and a Sphingomonas sp. strain NM05, on the degradation of γ-HCH in soil. Factors affecting degradation such as pH, incubation temperature and γ-HCH initial concentration were also studied. The results revealed that γ-HCH degradation efficiency is ~ 1.7–2.1 times greater in integrated system as compared to system containing either NM05 or CMC-Pd/nFe0 alone. The integration showed synergistic effect on γ-HCH degradation. Further, cell growth studies indicated that NM05 gets well acclimatized to nanoparticles, showing potential growth in the presence of CMC-Pd/nFe0 with respect to control system. This study signifies the potential efficacy of integrated technique to become an effective alternative remedial tool for γ-HCH contaminated soil. Further research in this direction could lead to the development of effective remediation strategies for other isomers of HCH and other chlorinated pesticides as well.

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