Abstract
A hybrid developed from a cross between two diverse Brassica napus cultivars (“Polo” and “Topas”) was used to produce a microspore derived double haploid (DH) population and a single seed descent derived recombinant inbred (RI) population for genetic mapping. Each of the two populations consisting of 190 DH lines and 94 RI lines was characterized for various types (SSR, SRAP, ISSR, SCAR) of polymorphic molecular markers. The DH population was scored for 620 molecular markers while the RI population was scored for 349 molecular markers to construct two independent genetic maps. In both genetic maps, all of the molecular markers were found to cluster in 19 linkage groups (LGs) covered a total genome length of 2244.1 cM and 1649.1 cM for the DH and RI maps, respectively. The data from the two genetic maps was used to construct a consensus integrated genetic map covering a total genome length of 2464.9 cM. Previously published Brassica reference genetic maps were used to assign each of the nineteen LGs to corresponding Brassica napus chromosomes named N01 to N19. To our knowledge, this is the first integrated genetic map based on DH and RI populations developed from the same cross in Brassica napus.
Highlights
Genetic maps of crop plants are considered standard tools or even “road maps” [1], to understand genome structure and organization and to tag economically important traits or genes
Two individual genetic maps were constructed with different types of markers, such as Simple Sequence Repeats (SSR), Sequence-related amplified polymorphism (SRAP), intersimple sequence repeat (ISSR) and sequence-characterized amplified region (SCAR)
Further these individual maps were combined into an integrated genetic map using double haploid (DH) and recombinant inbred (RI) populations in Brassica napus
Summary
Genetic maps of crop plants are considered standard tools or even “road maps” [1], to understand genome structure and organization and to tag economically important traits or genes Such maps are developed by following the inheritance of detectable markers or genes in segregating populations derived from crosses of diverse parents. The map was saturated using newly developed SSR markers designed from the information of SSR sequences in the gene bank and by using other marker types such as SRAP, ISSR, EST-SSR and SCAR These genetic maps will be a useful addition in understanding the Brassica napus genome and tagging the economically important genes in this important oil seed crop species. Linkage groups containing more than two common markers in each map were selected and integrated using the ‘Combine the Groups for Map Integration’ function
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