Abstract
A portable distance-based microfluidic aptasensor with sample-in-answer-out capability was designed for equipment-free, in-filed, visual quantitative detection of Salmonella by microcolumns-based vortex on-line micromixing and incubation, nitrocellulose membranes fixed with single-strand binding proteins (SSBP-NCMs)-based enrichment and separation, and distance-readout based visual quantitative detection. Specifically, aptamers modified with biotin (bio-aptamers) were used as detection probes and reacted with Salmonella typhimurium. The replaceable SSBP-NCMs with high adsorbability captured free bio-aptamers, making the microfluidic chips reusable. The formed bio-aptamer-SSBP-NCMs combined with horseradish peroxidase conjugated streptavidin (HRP-SA) which catalyzes hydrogen peroxide (H2O2) to produce oxygen (O2). O2 pushed the red gold nanoparticles (GNPs) to move a certain distance along serpentine channel. Hence, the readable distance signals were proportional to the concentrations of Salmonella in samples. The developed distance-based microfluidic aptasensor can detect Salmonella typhimurium as low as 3.7 × 101 cfu mL−1 and exhibited a good specificity and reliability.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
