An integrated and flexible PDMS/Au film-based electrochemical immunosensor via Fe–Co MOF as a signal amplifier for alpha fetoprotein detection

Abstract

Ultrasensitive determination of tumor marker (TM) is of great significance in cancer prevention and diagnosis. Traditional TM detection methods involve large instrumentation and professional manipulation, which complicate the assay procedures and increase the cost of investment. To resolve these problems, an integrated electrochemical immunosensor based on the flexible polydimethylsiloxane/gold (PDMS/Au) film with Fe–Co metal-organic framework (Fe–Co MOF) as a signal amplifier was fabricated for ultrasensitive determination of alpha fetoprotein (AFP). First, gold layer was deposited on the hydrophilic PDMS film to form the flexible three-electrode system, and then the thiolated aptamer for AFP was immobilized. Afterward, the aminated Fe–Co MOF possessing high peroxidase-like activity and large specific surface area was prepared by a facile solvothermal method, and subsequently the biofunctionalized MOF could effectively capture biotin antibody (Ab) to form MOF-Ab as a signal probe and amplify the electrochemical signal remarkably, thereby realizing highly sensitive detection of AFP with a wide linear range of 0.01–300 ng/mL and a low detection limit of 0.71 pg/mL. In addition, the PDMS based-immunosensor showed good accuracy for assaying of AFP in clinical serum samples. The integrated and flexible electrochemical immunosensor based on the Fe–Co MOF as a signal amplifier demonstrates great potential for application in the personalized point-of-care (POC) clinical diagnosis.