An Innovative HPTLC-based Approach for the Quantification of Withaferin A in Combinations of Herbal Compounds

Abstract

Withaferin A in herbal mixed dose forms was quantified utilizing a new destructive reagent that is quick, accurate, and economical. Toluene, ethyl acetate, and formic acid were mixed in a mobile phase with a ratio of 5:4:2 v/v/v, and separation was carried out at a detection wavelength of 254 nm. In accordance with ICH guidelines, the procedure was verified. A range of 90 to 630 ng/band was determined for the calibration. The method was validated in three separate experiments with varying concentrations of the standard additive. The limit of detection (LoD) of 2.43 ng and the limit of quantitation (LoQ) of 7.54 ng were noted. During system adaptability testing, %RSD was under 2. Using the same approach, unchanged, on nine different formulations proved the method’s separation and quantification efficiency