Abstract
Fluorescent dyes are of immense interest due to their nontrivial applications in cell imaging to observe different micro organelles and their functions. We have discovered organic Beet-root extracted water soluble fluorescent (BREWSF) dye as an efficient pigment for effective cell imaging. The extraction of this pigment from beet root and its preservation is very easy and cost effective. The BREWSF dye is characterized by IR, UV-Vis, NMR, mass, fluorescence spectroscopy and flowcytometry. By applying this dye on different types of human cells we obtained very good results in fluorescence microscopy, flowcytometry and confocal cell imaging. We also have noticed that this dye takes very less internalization time to produce very good fluorescence image and flowcytometry results of cells and also remains stable at different pH levels.
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