Abstract
A newly designed cryo-holder was built for the in-lens top stage of a Topcon DS-130 field emission scanning transmission and high resolution secondary electron-I microscope (STEM/HRSEM). The modified Oxford CT-3500 cryo-holder was designed to fit into the narrow bore in-lens of the DS-130F. The cryo-holder was designed to shuttle from the freezing chamber, to the high vacuum chamber of the Denton DV-602 magnetron sputter system for metal coating, and then to the interlock of the DS-130F upper stage. The intention was to provide a single cryo-holder to image frozen specimens by either HRSEM or STEM modes. In this electron optical design, many specimens, prepared by different cryo-preparation procedures, could be imaged by either or both imaging modes. The upper stage of the DS-130F was modified to accept a second liquid nitrogen (LN2) cold trap and a Varian cold cathode ion discharge gauge for monitoring the vacuum at the specimen (Fig. 1).
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