Abstract
We describe the detection characteristics of a device the Resonant Coil Magnetometer (RCM) to quantify paramagnetic particles (PMPs) in immunochromatographic (lateral flow) assays. Lateral flow assays were developed using PMPs for the measurement of total prostate specific antigen (PSA) in serum samples. A detection limit of 0.8 ng/mL was achieved for total PSA using the RCM and is at clinically significant concentrations. Comparison of data obtained in a pilot study from the analysis of serum samples with commercially available immunoassays shows good agreement. The development of a quantitative magneto-immunoassay in lateral flow format for total PSA suggests the potential of the RCM to operate with many immunoassay formats. The RCM has the potential to be modified to quantify multiple analytes in this format. This research shows promise for the development of an inexpensive device capable of quantifying multiple analytes at the point-of-care using a magneto-immunoassay in lateral flow format.
Highlights
Magnetic particles have many applications in biological research and are used very successfully as a solid phase to aid separation [1]
In this study we investigate the development of magneto-immunoassays in lateral flow format with paramagnetic particles (PMPs) as labels to quantify total prostate specific antigen (PSAT)
Assay variability in experimental quantitative lateral flow assays has been observed by others and indicates the challenge of using immunochromatography to provide quantitative data under research conditions [36]. In this investigation the aim of the study was to confirm that PMPs can be conjugated, used in lateral flow and that PMPs collected at capture lines can be quantified using the Resonant Coil Magnetometer (RCM)
Summary
Magnetic particles have many applications in biological research and are used very successfully as a solid phase to aid separation [1]. In immunoassays magnetic particles are used extensively as a solid phase e.g., Immulite Diagnostics Products Corporation, Siemens, Erlangen, Germany; FastPack, Qualigen, Carlsbad, CA and in molecular genetics in various kits for the isolation of nucleic acid. Magnetic particles have a number of advantages in use as labels in immunoassays including their stability and that they are not affected by the sample matrix. These properties are suitable for labels used in the development of point-of-care tests
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