Abstract
The cultivation of mycobacteria often requires the use of several antibiotics to limit the growth of other rapidly growing micro-flora present in the growth medium. This antibiotic cocktail is one of the most expensive reagents required for mycobacterium culture. Here we present a customized antibiotics mix that is easy to prepare at a fraction of the cost of the commercially available antibiotic mixture that protects against transient flora, which are normally present in lungs, without affecting mycobacterial colony number.
Highlights
Mycobacteria are slow-growing organisms (Lambrecht et al, 1988); to obtain visible colonies their culture has to continue for several days
Polymyxin B is a mix of polymyxin B1 and B2, basic polypeptides obtained from strains of Bacillus polymyxa
Preparation of Customized Antibiotics Mix (CAM) For the preparation of CAM, the aforementioned commercially available antibiotic formulations were procured and their working stocks were prepared in water
Summary
An inexpensive and easy-to-make customized antibiotics mix for mycobacterium culture [version 1; peer review: 2 approved].
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