Abstract
Abstract This paper reports on an industry friendly, inexpensive DNA extraction method for Penaeid shrimp that is compatible with Sequenom® iPLEX Platinum SNP pedigree genotyping platforms. The DNA extraction method employed a commercial prepGEM ™ insect DNA extraction kit (ZyGEM, Hamilton, New Zealand) in combination with water bath ultrasonic disruption. Two prepGEM ™ enzyme concentrations were compared; 100% and 10% of the manufacturer's recommendation. The utility of the method was compared to a more expensive DNeasy® extraction kit (QIAGEN, Hilden, Germany) by looking at DNA quality and yield from shrimp pleopod tips (approximately 3 mm 2 in size) and SNP call rates when DNA was run on a Sequenom® iPLEX Platinum pedigree genotyping panel. DNA from all methods (inexpensive and expensive) was of sufficient quality and quantity for multiplex PCR discrimination of SNP markers in a single Sequenom™ Platinum chemistry panel. This paper demonstrates that the inexpensive ZyGEM DNA extraction method will provide significant cost-savings to commercial operations that perform routine genotyping within breeding programs, and potentially broadening the use of DNA markers for commercial enterprises.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
