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Abstract
SUMMARY An indirect enzyme-linked immunosorbent assay (ELISA) is described for the detection of brucellosis in pigs. The combination of a commercial goat antirabbit peroxidase conjugate and an experimentally-prepared rabbit anti-porcine immunoglobulin serum detected both IgM and IgG antibodies to Brucella abortus in pigs. The test gives results comparable with the Coombs’ antiglobulin test but is more rapid and uses very small volumes of reagents.
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