An inactive chromatin configuration at the IL-10 locus in human neutrophils

Abstract

Event Abstract Back to Event An inactive chromatin configuration at the IL-10 locus in human neutrophils Nicola Tamassia1*, Maili Zimmermann1, Flavia Bazzoni1, Gioacchino Natoli2 and Marco A. Cassatella1 1 University of Verona, Department of Pathology and Diagnostic, Italy 2 European Institute of Oncology (IEO), Department of Experimental Oncology, Italy The ability of human neutrophils to express IL-10, a key cytokine whose function is to limit and terminate the inflammatory responses by regulating the expression of pro- and anti-inflammatory mediators, is currently controversial in the literature. In our hands, in fact, stimulation of highly purified populations of human neutrophils with diverse pathogen-associated molecular patterns (PAMPs), including Toll-like receptors (TLR) and C-type lectin receptor (CLR) agonists, or damage-associated molecular patterns (DAMPs), including serum amyloid protein A (SAA), do not trigger any mRNA expression or production of IL-10, differently from autologous monocytes and from studies of other groups. To identify the molecular basis of IL-10 expression in human phagocytes, we evaluated the chromatin modification status at their IL-10 genomic locus. By chromatin immunoprecipitation (ChIP) assays, we analyzed posttranslational modifications of histones associated with genes that are active, repressed or poised for transcriptional activation, including H3K4me3, H4Ac, H3K27Ac and H3K4me1 marks. Differently from autologous IL-10-producing monocytes, none of the marks under evaluation was detected at the IL-10 locus of resting or activated neutrophils. By contrast, elevated H3K4me3, H4Ac, H3K4me1 and H3K27Ac levels were detected at syntenic regions of the IL-10 locus in mouse neutrophils. Altogether, data demonstrate that human neutrophils, differently from either monocytes or mouse neutrophils, cannot switch on the IL-10 gene because its locus is in an inactive state, likely reflecting a neutrophil-specific developmental outcome. Implicitly, data also definitively settle a currently unsolved issue on the capacity of human neutrophils to produce IL-10. Keywords: IL-10, Neutrophils, Histone modificaitons, Monocytes, Chromatin Immunoprecipitation Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Tamassia N, Zimmermann M, Bazzoni F, Natoli G and Cassatella MA (2013). An inactive chromatin configuration at the IL-10 locus in human neutrophils. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00992 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Nicola Tamassia, University of Verona, Department of Pathology and Diagnostic, Verona, 37134, Italy, nicola.tamassia@univr.it Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Nicola Tamassia Maili Zimmermann Flavia Bazzoni Gioacchino Natoli Marco A Cassatella Google Nicola Tamassia Maili Zimmermann Flavia Bazzoni Gioacchino Natoli Marco A Cassatella Google Scholar Nicola Tamassia Maili Zimmermann Flavia Bazzoni Gioacchino Natoli Marco A Cassatella PubMed Nicola Tamassia Maili Zimmermann Flavia Bazzoni Gioacchino Natoli Marco A Cassatella Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.