Abstract
s1 May 1966An in Vitro Test of Cellular Immunity in Allergic Encephalomyelitis.George E. Seiden, M.D., F.A.C.P.George E. Seiden, M.D., F.A.C.P.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-64-5-1168_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptA sensitive method was developed for measuring cytotoxic effects produced by lymphoid cells harvested from rats with allergic encephalomyelitis. Allergic encephalomyelitis was produced by immunizing Lewis rats with 2.5 µg of Kies protein emulsified with Freund's complete adjuvant. Peritoneal cells were harvested 10 to 14 days after immunization. Harvested cells from specifically immunized rats were compared for cytopathic effect with equal numbers of control nonspecifically sensitized peritoneal cells. Cytopathic effect was measured by interacting lymphoid cells with HeLa or L cells, which had been grown in the presence of14C thymidine and subsequently "coated" with a solution of Kies protein... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Bronx, New York PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 May 1966Volume 64, Issue 5Page: 1168-1168KeywordsCellsEncephalomyelitisImmunityLymphocytesProteinsThymidines Issue Published: 1 May 1966 PDF downloadLoading ...
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