Abstract
BackgroundThe primary aim of this study was to observe the effect of 5-ALA-mediated photodynamic therapy on oral squamous cell carcinoma in vitro.MethodsSCC25 cells were divided into the observation group and the blank control group. Different concentrations of 5-ALA and SCC25 cells were co-incubated for different times, and the concentration of protoporphyrin IX was detected by flow cytometry. SCC25 cells were divided into the 5-ALA group (100 mg/L), the laser irradiation group alone, the 5-ALA plus laser irradiation group, and the blank control group (0 mg/L 5-ALA), and the methyl thiazolyl tetrazolium (MTT) solution method was used (each group was incubated for 4, 8 and 12 h in turn). The cell survival rate was calculated. Using annexin V-fluorescein isothiocyanate/propidium iodide method, the apoptosis of SCC25 cells was detected by flow cytometry.ResultsThe level of protoporphyrin IX in SCC25 cells increased with increased concentrations of 5-ALA and length of incubation. However, after 12 h, protoporphyrin IX level in SCC25 cells was gradually stabilized, and similar effect was obtained with 100 mg/L or more 5-ALA, indicating that the level of protoporphyrin IX in SCC25 cells was determined by 5-ALA concentration and incubation time. 5-ALA plus laser irradiation exerted an inhibitory effect on the growth of SCC25 cells, which was highly associated with drug dose and incubation time. Compared with the control group, laser irradiation alone or 5-ALA alone had no effect on the apoptosis of SCC25 cells. Different concentrations of 5-ALA combined with laser irradiation showed a remarkable effect of apoptosis, and a higher apoptosis rate was seen with higher drug concentrations.Conclusion5-ALA-mediated photodynamic therapy affects the growth of SCC25 cells in vitro, which may provide a new idea for the clinical treatment of oral squamous cell carcinoma.
Highlights
The primary aim of this study was to observe the effect of 5-aminolevulinic acid (5-ALA)-mediated photodynamic therapy on oral squamous cell carcinoma in vitro
The concentrations were set as 5 mg/L, 10 mg/L, 2 5 mg/L, 50 mg/L, 100 mg/L, 150 mg/L and 0 mg/L. 5-ALA and SCC25 cells were incubated for 2, 4, 8, 12 and 24 h, and the concentration of protoporphyrin IX in cells was detected by flow cytometry
After 12 h, the protoporphyrin IX level in SCC25 cells were gradually stabilized, and similar effect was obtained after using 100 mg/L or more 5-ALA, indicating that the level of protoporphyrin IX in SCC25 cells was determined by 5-ALA concentration and time
Summary
The primary aim of this study was to observe the effect of 5-ALA-mediated photodynamic therapy on oral squamous cell carcinoma in vitro. Photodynamic therapy is a therapeutic approach using photosensitive drugs combined with light irradiation to selectively destroy cancer cells under the effect of photodynamic process [3]. 5-aminolevulinic acid (5ALA) is the second-generation photosensitizer and an endogenous 5-carbon compound, which can produce strong photosensitive protoporphyrin IX. It can have photodynamic effect after laser irradiation. The current study was conducted to observe the effect of 5-ALA-mediated photodynamic therapy on OSCC in vitro, with an attempt to explore its underlying role and mechanism
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