Abstract
An in vitro permeation of a hydroalcoholic extract of Phyllanthus amarus (PaE) was investigated using excised human epidermis and shed king cobra skin as the barrier membranes. Donor and receptor compartments of diffusion cells were pH-controlled to simulate the permeation environment of the human skin. The PaE was analyzed by using normal-phase densitometric TLC detected at I» 280 nm and toluene:ethyl acetate (17:3) as the mobile phase. There were four major components observed in the saturated solution of the donor at pH 5.5. Over 24 h, only one component, possibly phyllanthin, was found in the receptor solution after permeation across the human epidermis, while two components, possibly phyllanthin and an another unknown permeated, permeated through shed snake skin. When compared to the saturated donor concentration, phyllanthin gave permeation fluxes of 0.04±0.01 and 0.12±0.02 %.cm-2.h-1 through the human and shed snake skins, respectively. It seems that only certain component(s) of the P. amarus extract could permeate through the skins, and by comparison, at a slower rate across the human skin than shed snake skin.
Highlights
Dermatological applications of herbal extracts for pharmaceutical and cosmetic purposes may have delivered some components which were not well documented
This study employed the human epidermis and shed snake skin as the barrier membranes and controlled the pH, which is likely to be one of the vital factors influencing the permeation of a hydroalcoholic extract of P.amarus (PaE)
DNA identification of P. amarus by sequence characterized amplified region (SCAR): Fresh leaves of P. amarus were used for genomic DNA extraction following a previously described method16
Summary
Dermatological applications of herbal extracts for pharmaceutical and cosmetic purposes may have delivered some components which were not well documented. Human skin is not as readily available as the waste material like shed snake skin, and both have been shown to give comparable in vitro permeation[9, 10, 11, 12]. This includes king cobras (Ophiophagus hannah) [12] which shed large pieces of skin readily usable for several experiments. This study employed the human epidermis and shed snake skin as the barrier membranes and controlled the pH, which is likely to be one of the vital factors influencing the permeation of a hydroalcoholic extract of P.amarus (PaE). The permeation profile studied by this type of herbal extract could provide a vital information for further product development
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: American Journal of Agricultural and Biological Sciences
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.