Abstract
1. 1. A radiometric micro-volume procedure for measurement of erythrocytic hexose monophosphate shunt (HMS) activity in intact cells in vitro is described. 2. 2. The procedure is rapid, allowing 200 individual HMS determinations in a single experiment of 5 hr duration. 3. 3. The procedure is reproducible, yielding HMS activity means insignificantly different ( P > 0.05) between replicate experiments. 4. 4. A profile of sodium nitrite-induced HMS stimulation is reported: HMS was elevated 2-fold ( P < 0.001) between zero and 2.5 mM NaNO 2; HMS elevation was more distinct (7-fold) between 2.5 and 5. 5.0mMNaNO 2; maximum activity (22-fold) was observed between 10 and 20mMNaNO 2; > 20mMNaNO 2 caused significant ( P < 0.001) diminution of HMS; glucose carbon recycling through the HMS occurred only with > 2.5mMNaNO 2 where this process contributed ⩽ 16% to total HMS activity.
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