Abstract
In this work, we described an in vitro system adequate for investigating the pathosystem soybean/arbuscular mycorrhizal fungi (AMF)/Fusarium virguliforme. Pre-mycorrhized plantlets with Rhizophagus irregularis were infected by F. virguliforme either locally via a plug of gel supporting mycelium (Method 1) or via a macroconidia suspension applied to the medium surface (Method 2). Root colonization by the AMF and infection by the pathogen were similar to the usual observations in pot experiments. Within a period of 18 days, more than 20% of the roots were colonized by the AMF and infection by the pathogen was observed in all the plants. In presence of AMF, a decrease in symptoms and in the level of root tissue infection was noticed. With Method 1, smaller necrotic lesions were observed in the pre-mycorrhized plantlets. In Method 2, pathogen infection was slower but more homogenous. These results demonstrated the suitability of the in vitro cultivation system to study the pathosystem soybean/AMF/F. virguliforme. We propose this in vitro cultivation system for studying the mechanisms involved in the biocontrol conferred by AMF against F. virguliforme in soybean.
Highlights
Studies on the belowground plant–microbes interaction have increased tremendously in number and complexity in recent years (Brink, 2016)
In the present study we reported and described, for the first time, an in vitro cultivation system associating pre-mycorrhized soybean plantlets infected by F. virguliforme
Root colonization by the arbuscular mycorrhizal fungi (AMF) was estimated 96 h (Method 1) and 72 h (Method 2) after inoculation by F. virguliforme. This corresponded to 19 (Method 1) and 18 (Method 2) days postplating of the soybean plantlets in the HC containing the extra-radical mycelium (ERM) of the AMF
Summary
Studies on the belowground plant–microbes interaction have increased tremendously in number and complexity in recent years (Brink, 2016). In the last decades, a number of cultivation systems have been developed to grow them in vitro with root organs (Gutjahr and Parniske, 2013) or whole plants (Voets et al, 2005, 2009; Dupré de Boulois et al, 2006; Koffi et al, 2009). These in vitro systems have allowed the in-deep study of the plant-AMF symbiotic association (Koffi et al, 2013)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
