An in vitro anther culture method for creating rice dihaploids resistant to prolonged flooding

Abstract

An assessment was made of the possibility of obtaining dihaploids by the method of anther culture in vitro to accelerate breeding for resistance to prolonged flooding of rice with water. The object of the study is F2 rice hybrids of the Federal State Budgetary Scientific Institution, Agrarian Research Center “Donskoy” (ARC “Donskoy”) rice breeding and seed laboratory, obtained by crossing the best varieties in terms of economically valuable traits with samples carrying genes for resistance to prolonged flooding with water. Basic nutrient media with the optimal composition of nutrients and growth hormones that stimulate callus and morphogenesis were used. Cultivation of anthers revealed large genotypic differences between the samples. In terms of responsiveness to neoplasms, 1/3 of the number of plants showed a positive result, the rest did not give calli. The most responsive to the formation of calli were hybrid combinations: 5009/2 – 84 pcs., 5010/2 – 94 pcs., 4565/3 –85 pcs., 4641/2 – 69 pcs. They also showed the ability to morphogenesis. Androgenic plants were obtained from 13 hybrid combinations, their share was 1.03% of the total number of inoculated anthers. 30 green regenerated lines were obtained from four rice hybrids, differing in visual morphological assessment: 5009/2 – 5 pcs., 5010/2 – 5 pcs., 4565/3 – 2 pcs., 4641/2 – 18 pcs. The isolated lines are characterized by good responsiveness in anther culture in vitro, carry genes for resistance to prolonged flooding, and can be used in rice breeding programs using DG technologies.