Abstract
An in vitro and in vivo characterization of the cadherin-catenin adhesion complex in a feline mammary carcinoma cell line
Highlights
Cadherins are members of a large family of transmembrane glycoproteins that mediate calcium-dependent homotypic cell-cell adhesion [1]
Cadherins connect indirectly to the actin cytoskeleton, through their cytoplasmic domain, via a group of proteins known as catenins [6]. β-Catenin is a 95 kDa protein that associates directly with cadherins, while α-catenin is a 102 kDa protein indirectly associated through interaction with β-catenin, mediating the interaction between cadherin-catenin complex (CCC) and actin cytoskeleton [7]. p120Catenin is a protein with multiple isoforms ranging in size from 90-120 kDa that binds to the cadherin juxtamembrane region, but does not link the complex to the actin cytoskeleton [7,8]
We search a feline mammary carcinomas (FMC) cell line in order to establish a suitable in vitro and in vivo model systems to evaluate the expression and functions of CCC molecules, namely P- and E-cadherin, as well as α, β, and p120-catenin, towards a better understanding of their role in feline mammary tumours progression. In this context we studied the feline mammary carcinoma cell line (FMCm), by evaluating the expression of the P-cadherin molecule as well as the other CCC molecules
Summary
Cadherins are members of a large family of transmembrane glycoproteins that mediate calcium-dependent homotypic cell-cell adhesion [1]. Cadherins connect indirectly to the actin cytoskeleton, through their cytoplasmic domain, via a group of proteins known as catenins [6]. Β-Catenin is a 95 kDa protein that associates directly with cadherins, while α-catenin is a 102 kDa protein indirectly associated through interaction with β-catenin, mediating the interaction between cadherin-catenin complex (CCC) and actin cytoskeleton [7]. Changes in the major molecules of the cadherin-catenin adhesion complex are related to the development of human breast cancer and their abnormal expression or function are associated to decreased intercellular adhesion, cell migration, invasion and metastatic dissemination [6,9,10]. Loss or delocalization of E-cadherin and catenins from the membrane is related to an invasive breast cancer phenotype [6]. P-cadherin overexpression is associated to breast cancer progression and worse patient survival [10,11]
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