Abstract
A possible and important sequalae of lung injury is the development of pulmonary fibrosis; however, conventional embedding procedures which use thick paraffm sections may not afford the pathologist the ability to identify an early fibrogenic response. Methacrylate-embedded tissue allows the histologist to section lungs at 1.0 micrometers, increasing the resolution for the identification of early histopathological changes. This latter method, in combination with a modification of a published trichrome staining method used to emphasize the pulmonary fibrotic response to an array of xenobiotics, is presented in this paper.
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