Abstract
Orthotolidine 1 was first proposed by E. B. Phelps 2 in 1909 as a qualitative test for minute amounts of free chlorine or hypochlorites in water. It is a crystalline basic body of the aromatic series obtained by reduction from orthonitrotoluene. It has a melting point of 129-130 centigrade and is quite insoluble in distilled water. It is quite soluble in acid solutions and in alcohol and ether. Ruttan and Hardisty 3 in 1912 described the use of orthotolidine as a test for the detection of blood. They used a 4 per cent solution in glacial acetic acid and added a small amount of perhydrol (Merck) as an oxidizing agent to obtain a blue color in the presence of blood. They stated that the solution would detect hemoglobin in a dilution of one part in 7,000,000 parts of water, in a dilution of one part in 24,000 parts of urine,
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