Abstract

The described method allowed good control of the degree of iodination which can be calculated fairly accurately if it is assumed that incorporation of carrier iodine parallels that of radioactive iodine. The possibility of denaturation or other damage to the protein is diminished by the use of an iodination solution free of oxidizing agents other than iodine itself. Using a crystalline glucagon preparation, incorporation of 15 to 25% of the totally employed radioactivity was obtained.

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