Abstract
SummaryThe standard method (protocol 1) for disinfecting shoot expiants used for initiation of plant tissue cultures failed when Iris rhizomes were infected with bacterial soft rot (Erwinia carotovora subsp, carotovora), Two new disinfection protocols were therefore developed, They involved keeping stock plants without irrigation for two months prior to excision of expiants; alcohol disinfection of the expiants prior to excision from the rhizome; dipping of the expiant in alcohol immediately after excision; soaking in 1 % (v/v) sodium hypochlorite (protocol 2) or saturated calcium hypochlorite (protocol 3); and soaking in mercuric chloride, With protocols 2 and 3 up to 90% and 62% respectively of expiants taken from infected Iris rhizomes showing soft-rot symptoms remained free of detectable microorganisms in vitro.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
