Abstract
A special chamber for thin-layer chromatography was devised in which compounds can be developed after adequate equilibration of the chromatographic media with the vapor of solvent systems. A comparison was made between the patterns of mobilities of several compounds when the compounds were developed using this chamber and a conventional chamber. Using the chamber, a well reproducible Rf value was obtained even when a compound was developed with the multicomponent solvent system and under different conditions of temperature.
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