An Improved Radioimmunoassay for Measurement of Pepsinogen in Porcine Blood Samples

Abstract

The study was conducted to develop a sensitive and specific radioimmunoassay (RIA) for the measurement of pepsinogen in porcine serum, and to use this test for the determination of pepsinogen concentrations in serum samples from fetuses and pigs of different ages. Compared to a previously described RIA, major improvements were made concerning the use of specific polyclonal antibodies and the use of an appropriate buffer. The assay was able to detect pepsinogen concentrations of ⩾0.2 ng/ mL . The recovery of pepsinogen was close to 95%. The intra-assay coefficients of variations ranged between 3.9 and 7.5% whereas the interassay ranged between 8.8 and 11.9%. These percentages correspond to a satisfactory accuracy and reproducibility of the assay. No cross-reactions were observed with the main commercially available products of the aspartic proteases family except porcine pepsin cross-reacted over 62.5 μg/ mL . Pepsinogen concentrations increased steadily with increasing age of the fetuses and the pigs ( P<0.05). Pepsinogen concentrations (±SE) in fetuses of 90–100 ( n=24) and 100–110 days of pregnancy ( n=36) were 0.5±0.1 and 5.3±1.3 ng/ mL , respectively. In pigs of 21, 98, and 213 days of age, the pepsinogen concentrations were 290.6±10.8, 343.1±17.9 and 383.5±15.3 ng/ mL , respectively. The results demonstrate that RIA is accurate and can be used easily to assess pepsinogen concentrations in pig sera. The test may constitute a valuable tool in epidemiological surveys and in studies related to gastric diseases in pigs.