Abstract
Gordonia are high GC gram-positive bacteria that have not yet been exploited well for biotechnological purposes because of the limited genetic tools. Described here is an improved protocol for electroporation, which is useful for several Gordonia species. The maximum transformation efficiency obtained was 2.8×104/μg (Gordonia rubropertinctus, Gordonia sp), and 1.7×103/μg (Gordonia amarae).
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